ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 161 PubMed citations. Promega AG wurde in der Schweiz 1991 unter dem Namen Catalys AG gegründet. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 177 PubMed citations. These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). These single-nucleotide overhangs anneal to the 3´‑thymidine overhangs of T-cloning vectors such as the Promega pGEM®-T (Cat.# A3600) and pGEM®-T Easy Vectors (Cat.# A1360) and the Invitrogen TOPO® TA Cloning Kit with pCR®2.1-TOPO® Vector (Invitrogen Cat.# K4500-01). On the basis of historical data, Reporter Gene Assay Kits market report provides key segments and their sub-segments, revenue and demand & supply data. (2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega. Store on ice until needed Transformation 1. Cloning - Promega pGEM-T Easy kit Ligation 1. Falls Sie über einen defekten Link hierher gekommen sind, schreiben Sie bitte eine E-Mail an den Webmaster (webmaster@promega.cc).Falls es … Vector Pgemt Easy, supplied by Promega, used in various techniques. Designed to work seamlessly with Promega assays, GloMax® microplate readers and luminometers come preloaded with protocols that allow you to run assays at the touch of a button. Choose from a range of DNA, RNA and protein molecular weight markers. Bioz Stars score: 99/100, based on 16 PubMed citations. e-mail: office@promega.cc homepage: www.promega.cc Filmenbuchgericht: Feldkirch Filmenbuch: FN 336296d UST-IdNr. Pgemt Promega Polylinker, supplied by Promega, used in various techniques. Pgemt Vector, supplied by Promega, used in various techniques. pGEM®-T and pGEM®-T Easy Vector Systems Technical Manual PDF (548 KB) – English. The luminescence signal from these reporter vectors is used as an indicator of the activity of any gene/DNA element fused to the reporter. DA: 59 PA: 43 MOZ Rank: 15. Contact Technical Services. Bioz Stars score: 99/100, based on 161 PubMed citations. Quick Protocols. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 99/100, based on 16 PubMed citations. www.promega.com Part# TM042 Printed in USA. 16, 9677–86. Cloning with pGEM®-T Vectors: Ligation; January 8, 2020; A Quick Method for A Tailing PCR Products; July 8, 2019; T-Vector Cloning: Questions, Answers and Tips ; November 12, 2019; The following two tabs change content below. Promega’s pGEM-T vector systems are very dependable and versatile tools to routinely clone fragments of DNA into reliable plasmids. Bioz Stars score: 99/100, based on 177 PubMed citations. Promega AG beschäftigt 16 Mitarbeiter. • Construction of unidirectional nested deletions with the Erase-a-Base® System. A protocol for isolation of genomic DNA from white blood cells, tissue culture cells, animal and plant tissue, yeast, Gram-positive and Gram-negative bacteria. Literature # TM040. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. 5 µl 2x Rapid Ligation Buffer c. 1 µl pGEM-T vector d. 1 µl T4 DNA Ligase 2. Video Protocols. The product portfolio includes RNA purification solutions for all sample throughput needs, from manual purification to high-throughput automated systems. Technical Manual pGEM®-T and pGEM®-T Easy Vector Systems カタログカタログカタログ番号カタログ番号番号番号 A1360, A1380, A3600, A3610 www.promega.co.jp 注意注意注意：注意：：： この日本語マニュアルは製品に添付される英文マニュアルを翻訳したもの and Graham, A. • Construction of unidirectional nested deletions with the Erase-a-Base® System. The pGEM-T and pGEM-T Easy plasmid vectors are essentially the same but with one important difference. • Blue/white screening for recombinants. • In vitro transcription from dual-opposed promoters. pGEMt_vector - Free download as PDF File (.pdf), Text File (.txt) or read online for free. Get cells out of freezer, thaw on ice for 5 minutes 2. 3 µl Fresh PCR product b. プロトコル クイックプロトコル (pGEM-T Vectors) 製品マニュアル. Complete Protocol. Instructions for Use of Product(s) M7121: GoTaq Green Master Mix, M7122, M7123 Literature # 9PIM712. Add to the reaction mixture: a. Die von Ihnen gewünschte Seite kann nicht gefunden werden, oder ist momentan nicht erreichbar. Protocols. Promega RNA purification products extract high-quality total RNA, mRNA or miRNA from many sample types, including blood, cells, tissues, FFPE tissue and total RNA preparations. Pgemt, supplied by Promega, used in various techniques. Dual-Luciferase Reporter Assay and Dual-Luciferase Reporter 1000 Assay System Quick Protocol FB024 PDF (73 KB) – English. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 2 PubMed citations. • Production of ssDNA. Protein expression vectors are basic tools for production of proteins. プロメガ（Promega Corporation）はライフサイエンスの分野に生化学および分子生物学に関連する革新的で価値の高い製品を世界の研究者にお届けするリーディングカンパニーです。 Eukaryotische Expressionssysteme Analyse der Top-Unternehmen 2021 – Thermo Fisher, Agilent, New England Biolabs, Sigma Aldrich, Promega apexresearch März 9, 2021 Nischenmarktforschungsbericht 2021 über Eukaryotische Expressionssysteme Markt mit Branchenwachstumsanalyse, Wettbewerb, Trends und Prognose bis 2026. ZERO BIAS - scores, article reviews, protocol conditions and more Bioz Stars score: 97/100, based on 572 PubMed citations. Promega Corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, high-value products for the life sciences. • Production of ssDNA. • In vitro transcription from dual-opposed promoters. • Blue/white screening for recombinants. Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3600; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. These single 3«-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmid. Specifications. Company Promega; Price Supplier Page View Company Product Page; Catalog Number A3610; Quantity 20 reactions; Vector Name pGEM®-T Vector; Applications • Cloning PCR products. You are viewing: E1910 Change Configuration. 製品マニュアル（日本語） DH5α使用説明書. Newton, C.R. The Promega mission statement is: To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Bioz Stars score: 97/100, based on 572 PubMed citations. Pgemt Easy Vector, supplied by Promega, used in various techniques. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. Instructions for Use of Product(s) E1910, E1960. Die Promega … Luminometer and Microplate Reader Selection Guide. (1988) Nucl. Clark, J.M. ベクターマップ＆シークエンス Robles, J. and Doers, M. (1994) Promega Notes 45, 19–20. Incubate at room temperature for 1 hour or overnight at 4°C 3. ATU65297145 ARA -Nummer: 17418 PROMEGA Handelsgesellschaft m.b.H Treietstraße 1 A-6833 Klaus Page 4 Revised 5/07 GGAGA GCTCC CAACG CGTTG GATGC ATAGC TTGAG TATTC TATAG … Related Posts. Bioz Stars score: 97/100, based on 2 PubMed citations. What's in the box? The pGEM-T Vector is prepared by cutting Promega's pGEM-5Zf(+) Vector with EcoR V and adding a 3« terminal thymidine to both ends. Item Part # Size Available Separately; Luciferase Assay Substrate. Read More About Sensitivity. E151A: 1 × 1 vial: Passive Lysis Buffer, 5X. The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual-reporter assays. Promega Technical Services Scientists are here to assist you in troubleshooting your experiments at any time. Theoretically, smaller inserts will be cloned more efficiently than longer inserts. “Reporter Gene Assay Kits Market Scenario 2020-2027:. The pGEM®-9Zf(–) Vector is a recombinant plasmid designed to provide a versatile range of cloning strategies, and contains T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of β-galactosidase. Als Tochtergesellschaft von Promega Corp. bietet die Schweizer Niederlassung in Dübendorf zusätzlich und komplementär zu Produkten von Promega Corp. auch Labor- und Analysegeräte sowie Verbrauchsmaterialien der Firmen Serva GmbH an. Many Promega Vectors are reporter vectors containing various combinations of luciferase genes (firefly, Renilla, NanoLuc luciferase) with other genetic elements. 1/21 Apoptosis Assays ProductSize Cat.# Caspase-Glo® 3/7 Assay System 2.5ml G8090 Caspase-Glo® 3/7 3D Assay System 10ml G8981 RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay 100 assays JA1011 Additional … The pGEM-T Easy vector has EcoRI restriction sites surrounding the proposed insert site, whereas the pGEM-T vector does not. Seite nicht gefunden. Use the DNA molecular weight markers for both conventional and pulsed field gel electrophoresis applications. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions. Schweden. The Global Reporter Gene Assay Kits market exhibits comprehensive information that is a valuable source of insightful data for business strategists during the decade 2014-2027. The pGEM-T and pGEM-T Easy Systems now allow you to perform ligation reactions in as little as one hour. Acids Res. (1994) In: PCR, BIOS Scientific Publishers, Ltd., Oxford, UK, 13. GoTaq® Green Master Mix is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl 2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. Purified DNA is suitable for amplifications, restriction enzyme digestion and membrane hybridizations (e.g., Southern and dot/slot blots).